Research Tool
Calculate the concentration of a peptide stock solution. Enter the peptide mass in the vial and the volume of diluent added; the result is shown in mg/mL and µg/mL.
Calculators for a specific compound:
Total mg of lyophilized peptide in the vial
Volume of diluent added to the vial
Concentration
5
mg/mL (equal to µg/µL)
Same concentration
5,000
µg/mL
Concentration (mg/mL) = peptide mass (mg) ÷ diluent volume (mL). For in-vitro laboratory research use only.
Concentration is the peptide mass divided by the diluent volume: a 10 mg vial dissolved in 2 mL gives 5 mg/mL, which is the same as 5,000 µg/mL or 5 µg/µL.
The mass in the vial does not change, only how it is distributed: doubling the volume halves the concentration, and halving it doubles the concentration.
Lyophilized peptides are dissolved in a diluent suited to the compound's solubility and to the assay, such as sterile water, a buffer, or bacteriostatic water (sterile water with 0.9% benzyl alcohol as a preservative).
Check the compound's published solubility data and the product's Certificate of Analysis before choosing a diluent and volume.
Label each stock solution with the compound, the lot number from the vial, the concentration, the diluent and the preparation date, so results can be traced back to the lot's Certificate of Analysis.
Keep lyophilized powder at -20°C. Peptide solutions are generally held at 2–8°C and protected from light; avoid repeated freeze-thaw cycles, which degrade peptide integrity. Refer to the product's Certificate of Analysis and the published literature for compound-specific stability.
Research Use Only. This calculator is provided as a convenience tool for qualified researchers. All compounds sold by Purely Peptides are intended for in-vitro laboratory use only. Not for human or veterinary use.