Research Tool
Enter vial mass, diluent volume and the amount you want per draw to get the concentration, syringe units and draws per vial.
Total mg of lyophilized peptide in the vial
Volume of diluent added to the vial
The amount you want in each syringe draw
Syringe size
Concentration
5
mg/mL (equal to µg/µL)
Same concentration
5,000
µg/mL
Concentration (mg/mL) = peptide mass (mg) ÷ diluent volume (mL). For in-vitro laboratory research use only.
Enter an amount per draw to see the syringe units, volume and draws per vial.
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Record the compound, mass, diluent volume, concentration, date prepared and optional lot.
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Concentration is the vial mass divided by the volume of bacteriostatic (BAC) water, in mg/mL. Syringe units are hundredths of a millilitre on every syringe size, so 10 units is 0.1 mL on a U-100, U-50 or U-30 syringe, and the mg in 10 units is the concentration times 0.1. These are arithmetic examples, not preparation recommendations.
| Vial | BAC water | mg/mL | mg in 10 units, U-100 (1 mL) | mg in 10 units, U-50 (0.5 mL) | mg in 10 units, U-30 (0.3 mL) |
|---|---|---|---|---|---|
| 5 mg | 1 mL | 5 | 0.5 | 0.5 | 0.5 |
| 5 mg | 2 mL | 2.5 | 0.25 | 0.25 | 0.25 |
| 5 mg | 3 mL | 1.67 | 0.17 | 0.17 | 0.17 |
| 5 mg | 5 mL | 1 | 0.1 | 0.1 | 0.1 |
| 10 mg | 1 mL | 10 | 1 | 1 | 1 |
| 10 mg | 2 mL | 5 | 0.5 | 0.5 | 0.5 |
| 10 mg | 3 mL | 3.33 | 0.33 | 0.33 | 0.33 |
| 10 mg | 5 mL | 2 | 0.2 | 0.2 | 0.2 |
| 15 mg | 1 mL | 15 | 1.5 | 1.5 | 1.5 |
| 15 mg | 2 mL | 7.5 | 0.75 | 0.75 | 0.75 |
| 15 mg | 3 mL | 5 | 0.5 | 0.5 | 0.5 |
| 15 mg | 5 mL | 3 | 0.3 | 0.3 | 0.3 |
| 30 mg | 1 mL | 30 | 3 | 3 | 3 |
| 30 mg | 2 mL | 15 | 1.5 | 1.5 | 1.5 |
| 30 mg | 3 mL | 10 | 1 | 1 | 1 |
| 30 mg | 5 mL | 6 | 0.6 | 0.6 | 0.6 |
Concentration is the peptide mass divided by the diluent volume: a 10 mg vial dissolved in 2 mL gives 5 mg/mL, which is the same as 5,000 µg/mL or 5 µg/µL.
The mass in the vial does not change, only how it is distributed: doubling the volume halves the concentration, and halving it doubles the concentration.
Lyophilized peptides are dissolved in a diluent suited to the compound's solubility and to the assay, such as sterile water, a buffer, or bacteriostatic water (sterile water with 0.9% benzyl alcohol as a preservative).
Check the compound's published solubility data and the product's Certificate of Analysis before choosing a diluent and volume.
Label each stock solution with the compound, the lot number from the vial, the concentration, the diluent and the preparation date, so results can be traced back to the lot's Certificate of Analysis.
Keep lyophilized powder at -20°C. Peptide solutions are generally held at 2–8°C and protected from light; avoid repeated freeze-thaw cycles, which degrade peptide integrity. Refer to the product's Certificate of Analysis and the published literature for compound-specific stability.
Research Use Only. This calculator is provided as a convenience tool for qualified researchers. All compounds sold by Purely Peptides are intended for in-vitro laboratory use only. Not for human or veterinary use.