Compound monograph
SHLP6
SHLP6 is a 20-residue peptide assigned to the mitochondrial 16S rRNA region and distinguished from other SHLP members by its sequence and reported cellular response.
01
Identity & nomenclature
SHLP6 is a 20-residue peptide assigned to the mitochondrial 16S rRNA region and distinguished from other SHLP members by its sequence and reported cellular response.
SHLP6 is twenty residues; descriptions that give all SHLPs a common length range are wrong. It is neither a truncated humanin nor interchangeable with SHLP2 or SHLP3, and it does not behave like them — see structuralNotes.
Canonical name: Small humanin-like peptide 6.
Declared aliases and development codes: SHLP-6.
02
Molecular properties
| Molecular formula | C107H177N27O30S2 |
|---|---|
| Molecular mass | 2385.9 Da |
| One-letter sequence | MLDQDIPMVQPLLKVRLFND |
Twenty residues, free at both termini. TWO methionines, at positions 1 and 8, and NO cysteine — so it carries two sulfur atoms without any thiol.
03
Structural characteristics
The discovery comparison found different cellular responses across the SHLP series: SHLP6 increased apoptosis in the assay where SHLP2 and SHLP3 reduced it. Family membership does not establish a common cellular effect. Separately, SHLP6 and humanin showed strong synonymous codon bias and sequence conservation, consistent with amino-acid sequence constraint. This support does not extend to SHLP2 or SHLP3. No direct receptor or molecular target is established here.
Terminal features: Free N-terminus, Free C-terminal carboxyl.
04
Molecular targets & mechanisms
05
Analytical considerations
Fragmentation should cover methionine 8 and the C-terminal aspartate. With two methionines, singly and doubly oxidised species are relevant identity impurities rather than incidental ones. The peptide contains no cysteine, so any proposed disulfide structure contradicts this sequence outright. Antibody detection and coding-sequence conservation each establish something real and neither establishes intact endogenous termini, which remain unknown.
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Verified bibliography
- Naturally occurring mitochondrial-derived peptides are age-dependent regulators of apoptosis, insulin sensitivity, and inflammatory markers.current
Cobb LJ, Lee C, Xiao J, Yen K, Wong RG, Nakamura HK, Mehta HH, Gao Q, Ashur C, Huffman DM, Wan J, Muzumdar R, Barzilai N, Cohen P
Aging · 2016-04 · Journal Article
- Evidence of natural selection in the mitochondrial-derived peptides humanin and SHLP6.current
Gruschus JM, Morris DL, Tjandra N
Scientific reports · 2023-08-29 · Journal Article
- The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance.current
Lee C, Zeng J, Drew BG, Sallam T, Martin-Montalvo A, Wan J, Kim SJ, Mehta H, Hevener AL, de Cabo R, Cohen P
Cell metabolism · 2015-03-03 · Journal Article
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Methodology & citation verification
Reference identity is checked against official PubMed metadata. Local deterministic receipts bind each normalized title and canonical metadata record to its verification date. Scientific values remain absent when an authoritative source has not been verified.
Read the full methodology →