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Purely Peptides
Research Catalog

Compound monograph

HNG ([Gly14]-Humanin)

HNG is a synthetic single-substitution analog of humanin carrying glycine in place of serine at position 14.

Published: 2026-09-16Literature/identifier verification: 2026-09-15
HNGS14G-humaninS14G-HNHumanin-GMitochondrial-derived peptides

01

Identity & nomenclature

HNG is a synthetic single-substitution analog of humanin carrying glycine in place of serine at position 14.

HNG, S14G-HN and [Gly14]-humanin name the same substitution. 'Humanin-G' denotes substitution at position 14, not a glycine extension. HNGF6A additionally replaces Phe6 with alanine. These synthetic analogs require sequence-specific attribution; mitochondrial family membership does not establish endogenous expression.

Canonical name: [Gly14]-humanin.

Declared aliases and development codes: HNG, S14G-humanin, S14G-HN, Humanin-G.

02

Molecular properties

Molecular formulaC118H202N34O31S2
Molecular mass2657.2 Da
CAS Registry Number330936-70-4
PubChem CID16135997
One-letter sequenceMAPRGFSCLLLLTGEIDLPVKRRA

Twenty-four residues, free at both termini. Identical to humanin except at position 14, where serine is replaced by glycine.

03

Structural characteristics

Cell-binding experiments tested biotin-labeled humanin and HNG separately; receptor-subunit knockdown reduced HNG binding, and reconstituted-protein assays examined both peptides. In a separate biophysical study, HNG and SHLP2 associated with misfolded IAPP seeds. Fluorescence and co-sedimentation supported seed binding, while EPR and circular dichroism did not support binding to IAPP monomers. Rodent disposition experiments compared HNG with HNGF6A and included an IGFBP-3-deficient group; plasma measurements used a humanin ELISA, so immunoreactivity is distinct from intact-peptide identification.

  • S14G

Terminal features: Free N-terminus, Free C-terminal carboxyl.

04

Molecular targets & mechanisms

05

Analytical considerations

Verify glycine at position 14 with fragments spanning it. Relative to humanin the substitution removes CH2O, a calculated monoisotopic difference of 30.011 Da — small, and a fragment that omits position 14 cannot distinguish the two molecules at all. A humanin immunoassay or a shared biological response cannot establish which analog was supplied.

06

Verified bibliography

  1. Humanin inhibits neuronal cell death by interacting with a cytokine receptor complex or complexes involving CNTF receptor alpha/WSX-1/gp130.

    Hashimoto Y, Kurita M, Aiso S, Nishimoto I, Matsuoka M

    Molecular biology of the cell · 2009-04-22 · Journal Article

    current
    PMID 19386761PMC2695794DOI 10.1091/mbc.e09-02-0168Humanin (HN)HNG ([Gly14]-Humanin)
  2. Pharmacokinetics and tissue distribution of humanin and its analogues in male rodents.

    Chin YP, Keni J, Wan J, Mehta H, Anene F, Jia Y, Lue YH, Swerdloff R, Cobb LJ, Wang C, Cohen P

    Endocrinology · 2013-07-08 · Journal Article

    current
    PMID 23836030PMC3776863DOI 10.1210/en.2012-2004HNG ([Gly14]-Humanin)HNGF6A
  3. The Mitochondrial-Derived Peptides, HumaninS14G and Small Humanin-like Peptide 2, Exhibit Chaperone-like Activity.

    Okada AK, Teranishi K, Lobo F, Isas JM, Xiao J, Yen K, Cohen P, Langen R

    Scientific reports · 2017-08-10 · Journal Article

    current
    PMID 28798389PMC5552803DOI 10.1038/s41598-017-08372-5HNG ([Gly14]-Humanin)SHLP2

10

Methodology & citation verification

Reference identity is checked against official PubMed metadata. Local deterministic receipts bind each normalized title and canonical metadata record to its verification date. Scientific values remain absent when an authoritative source has not been verified.

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